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Dade Behring diffquick dade behring staining
Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
Diffquick Dade Behring Staining, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dade+diffquick/diffquick+staining+solution/pmc07064269-14-33-34
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1) Product Images from "Bacillus subtilis exopolysaccharide prevents allergic eosinophilia."

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

Journal: Allergy

doi: 10.1111/all.13674

Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
Figure Legend Snippet: Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Techniques Used: Staining

Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.
Figure Legend Snippet: Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Techniques Used: Incubation, Generated, Staining

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Incubation:

Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy.
Article Snippet: .. After 24 h of incubation at 37°C, the upper surfaces of the upper chambers were gently wiped with a cotton swab, and the invading cells were subsequently fixed and stained using a DiffQuick cell staining kit (Dade Behring). ..



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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Image Search Results


Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Journal: Allergy

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

doi: 10.1111/all.13674

Figure Lengend Snippet: Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Article Snippet: Following the final HDM treatment, bronchial alveolar lavage (BAL) was collected from euthanized mice, by flushing the lungs with 0.8mL PBS containing 10% FCS, 1mM EDTA) and immune cell infiltration was assessed by DiffQuick (Dade Behring) staining of cytospun cells ( ).

Techniques: Staining

Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Journal: Allergy

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

doi: 10.1111/all.13674

Figure Lengend Snippet: Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Article Snippet: Following the final HDM treatment, bronchial alveolar lavage (BAL) was collected from euthanized mice, by flushing the lungs with 0.8mL PBS containing 10% FCS, 1mM EDTA) and immune cell infiltration was assessed by DiffQuick (Dade Behring) staining of cytospun cells ( ).

Techniques: Incubation, Generated, Staining